METHOD OF CULTURE DIAGNOSIS OF TUBERCULOSIS INFECTION Russian patent published in 2017 - IPC C12Q1/04 C12N1/20 C12R1/32 

FIELD: biotechnology.

SUBSTANCE: invention relates to biotechnology. Method involves following. Collected sputum is subjected to fast freezing at temperature from -20 to -25 °C without preserving agents and kept at this temperature from 16 to 24 hours. It is unfrozen at room temperature and treated with 4 % solution of sodium hydroxide containing N-acetyl-L-cysteine during 20-25 minutes. Sodium phosphate buffer 1 M is added and mixture is centrifuged at 3,000–3,100 g for 20–25 minutes. Sample is decanted to separate precipitate. Iron oxide is added to precipitate in final concentration of 0.04 M, while stirring, in sodium phosphate buffer 1 M, pH 6.8 and inoculated on nutrient medium Lowenstein-Jensen.

EFFECT: invention allows reducing time for obtaining positive results of dense nutrient medium.

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