METHOD FOR DETECTING MYCOBACTERIUM TUBERCULOSIS FROM RESECTED LUNG TISSUE Russian patent published in 2021 - IPC G01N33/48 

Abstract RU 2759413 C1

FIELD: medicine; microbiology.

SUBSTANCE: invention relates to medicine, namely to microbiology; it can be used for laboratory diagnostics of tuberculosis. The essence of a method for detecting mycobacterium tuberculosis from resected lung tissue is that: test material is processed from accompanying microflora by placing test material for 45 min in a 0.9% ozonated NaCl solution heated to a temperature of 21°C, which is continuously saturated with an ozone-oxygen mixture with an ozone concentration of 10,000 mcg/l /min; after processing material, it is centrifuged at a speed of 3000 rpm for 15-20 min; supernatant liquid is drained, precipitation is sown equally in test tubes with nutrient media.

EFFECT: claimed method allows one to increase the accuracy of laboratory diagnostics in the detection of mycobacterium tuberculosis while reducing the time of laboratory tests by preserving the viability and reducing quantitative losses of mycobacteria in test material.

1 cl, 1 ex

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RU 2 759 413 C1

Authors

Dotsenko Ivan Aleksandrovich

Chertkov Aleksandr Kuzmich

Skornyakov Sergej Nikolaevich

Medvinskij Igor Davydovich

Golubeva Lyudmila Andreevna

Raevskaya Natalya Vasilevna

Leshchinkova Anna Viktorovna

Gusejnova Kamila Alievna

Isabekova Patimat Shamilevna

Izmodenova Mariya Yurevna

Berdnikov Roman Borisovich

Dates

2021-11-12Published

2018-10-08Filed