RECOMBINANT PROTEIN Mio-HSP, METHOD OF ITS PRODUCTION, INJECTION PREPARATION FOR MUSCLE MASS INCREASE IN FARM ANIMALS, BIRDS AND ANIMALS OF CANIDS, AS WELL AS METHOD OF USING PREPARATION Russian patent published in 2017 - IPC C07K19/00 C07K14/475 A61K38/18 A61P21/00 C12P1/00 

FIELD: biotechnology.

SUBSTANCE: present invention relates to biotechnology and can be used for increasing muscle mass of farm animals, livestock and canines. Fused protein is obtained, consisting of myostatin, Gli-Ser spacer and glucan binding domain of alpha-glucan binding domain of Streptococcus mutans (Mio-HSP), that is used to produce injection preparation for subcutaneous or intramuscular injections in a dose of 50–200 mcg of said protein per one kg of animal body weight or poultry. For producing recombinant protein is grown cell strain E. coli M15 [rMio-HSP] - producer of recombinant protein myocardial HSP, which is obtained by transformation of cells of E. coli M15 plasmid rMio-HSP M15 containing a nucleotide sequence gene coding myocardial HSP, then bind protein myocardial HSP in paragraph 1 in the cell extracts strain E. coli M15 [rMio-HSP] with alpha-glucan binding sorbent by affine interaction incubation procedure, with further washing from unbound bacterial proteins and separating end product.

EFFECT: invention allows to effectively induce synthesis of specific antibodies to myostatin, inhibit and, consequently, stimulate growth of muscular tissue.

4 cl, 7 tbl, 6 ex