METHOD FOR PREPARING CONJUGATE OF HYALURONIDASE WITH DERIVATIVES OF POLYETHYLENE PIPERAZINE AND APPLICATION OF PRODUCED CONJUGATE Russian patent published in 2017 - IPC A61K9/02 A61K9/06 A61K31/787 A61K38/43 A61P19/04 A61P29/00 C08F8/02 C08F8/06 C08F26/06 

FIELD: pharmacology.

SUBSTANCE: group of inventions relates to medicine, namely, to the process of preparing an active conjugate of hyaluronidase enzyme with copolymer using a carbodiimide method or azide method of conjugation, purification, concentration and freeze-drying (or dilution). The copolymer is a water soluble copolymer of N-oxide of 1,4-ethylene piperazine, (N-carboxymethyl)-1,4-ethylene piperazinium or its hydrazide, and 1,4-ethylene piperazine of the general formula:

,

where n is from 40% to 90% of the total units, m is from 3% to 40% of the total units, n+m+1=100% derived from poly-1,4-ethylene piperazine by oxidation, alkylation and, in the case of an azide method, hydrazinolysis. Also a drug having properties of suppressing hyperplasia of the connective tissue and anti-inflammatory effect is disclosed. It is obtained by one of the embodiments of the method in the form of a suppository, ointment, injection or cosmetic product.

EFFECT: increased hyaluronidase activity, degree of conjugation, yield, drug storage stability.

15 cl, 10 ex, 3 tbl, 2 dwg