FIELD: medicine.
SUBSTANCE: paraquat at a concentration of 0.25-1.0 mM is added to the fibroblasts culture with a density of 2-5×103 cells in a volume of 100 mcl, and kept for 48 hours, or at a concentration of 5 mM, and held for 24 hours, then stained with resazurin, the amount of live fibroblasts is determined from the change in the resazurin fluorescent signal, and, in the presence of 50% or more live fibroblasts, as compared to healthy human fibroblasts, Leber's hereditary optical atrophy is diagnosed.
EFFECT: increased accuracy of disease diagnosis for correct treatment, prediction of the disease course in a proband, prediction for proband relatives and offspring, simplification and reduced cost of the method, diagnostic process acceleration and a possibility of using the method for a large number of cell lines.
4 dwg, 3 ex
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