FIELD: biotechnology.
SUBSTANCE: biological material is collected and DNA is released. PCR is performed for mitochondrial DNA cytochrome oxidase section. As a primers, direct EuCO1F is used: GAATATGAGCCGGAATAGTAGGA (SEQ ID NO: 3) and reverse EuCO1R: ATGTGTTGAAGTTACGGTCA (SEQ ID NO: 4). PCR products were subjected to restriction. As restrictase, the following are used: Ahl I, BamH I, Bst2U I, Psi I. Restriction products visualization is performed with the by electrophoresis in 2% agarose gel. The presence of one of fragments sets: 317, 268 bp for restriction Ahl I, 471, 114 bp for BamH I, 364, 221 bp for Bst2U I and 435, 150 bp for Psi I indicates the pest belonging to the Eurygaster integriceps genus.
EFFECT: invention allows, during period of from 4,5 to 6 hours, to carry out identification Eurygaster integriceps at different stages of its development and regardless of insect gender.
2 dwg, 1 ex
| Title | Year | Author | Number |
|---|---|---|---|
| METHOD FOR DIFFERENTIATION OF COMMERCIALLY IMPORTANT MITES OF AMBLYSEIUS GENUS BASED ON RESTRICTIVE ANALYSIS | 2016 |
|
RU2631933C1 |
| METHOD FOR CARRYING OUT PCR WITH ALLELE-SPECIFIC PROBES FOR GENOTYPING CATTLE BY THE ALLELES A AND K OF THE DGAT1 GENE | 2018 |
|
RU2662972C1 |
| METHOD FOR IDENTIFICATION OF SIGNIFICANT FOR AGRICULTURE REPRESENTATIVES OF GENUS BACILLUS-BACILLUS SUBTILIS, BACILLUS MEGATERIUM AND BACILLUS THURINGIENSIS BASED ON RESTRICTION ANALYSIS OF GENE 165S RRNA | 2023 |
|
RU2822737C1 |
| METHOD FOR IDENTIFYING IMPORTANT FOR AGRICULTURE FUNGI OF THE GENUS TRICHODERMA-TRICHODERMA HARZIANUM, TRICHODERMA VIRIDE AND TRICHODERMA ATROVIRIDE BASED ON PCR-RFLP | 2023 |
|
RU2822738C1 |
| METHOD OF DIFFERENTIATION OF KEY BREEDS OF HONEY BEES IN RUSSIA BASED ON MUTAGENIC PCR-RFLP | 2017 |
|
RU2653435C1 |
| METHOD FOR PREDICTION OF RISK OF RENAL CELL CARCINOMA | 2019 |
|
RU2721709C1 |
| DIAGNOSTIC TECHNIQUE FOR PREDISPOSITION TO RENAL CELL CARCINOMA BASED ON PCR-RFLP | 2019 |
|
RU2723090C1 |
| METHOD OF CARRYING OUT PCR-RFLP FOR GENOTYPING CATTLE ON ALLELES A AND K OF GENE DGAT1 | 2013 |
|
RU2528743C1 |
| METHOD FOR CREATING WHEAT PLANTS RESISTANT TO EURYGASTER INTEGRYCEPS PUTON | 2004 |
|
RU2277586C2 |
| METHOD OF IDENTIFICATION OF SPECIES OF PREDATORY MITES OF AMBLYSEIUS GENUS | 2023 |
|
RU2804853C1 |
