FIELD: biotechnology.
SUBSTANCE: recombinant baculovirus for recombinant protein expression, a transfer vector, a cloning vector and a nucleic acid sequence suitable for obtaining of the said baculovirus, and application there of are proposed, as well as a host cell and a method for recombinant protein production. The proposed recombinant baculovirus contains a nucleic acid sequence, which provides IE-1 or IE-0 protein expression at a level greater than the endogenous level, recombinant homologous region as an enhancer region operable linked to a promoter and a nucleic acid sequence encoding the recombinant protein. Thestructure of the proposed baculovirus provides an increased expression level of the target recombinant protein.
EFFECT: group of inventions can be used for production of recombinant proteins using baculovirus vector expression systems in insect cells.
17 cl, 10 dwg, 1 tbl, 16 ex
| Title | Year | Author | Number |
|---|---|---|---|
| AVV VECTORS WITH IMPROVED Rep-CODING SEQUENCES USED IN INSECT CELL PRODUCTION SYSTEMS | 2007 |
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| METHODS FOR ENHANCING BIOLOGICAL ACTIVITY OF RECOMBINANT ADENO ASSOCIATED VIRUS PRODUCED BY BACULOVIRUS SYSTEM | 2017 |
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| RECOMBINANT EXPRESSION OF PROTEIN ORF2 PCV2b IN INSECT CELLS | 2018 |
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| RECOMBINANT NONPATHOGENIC MDV VECTOR PROVIDING POLY-SPECIFIC IMMUNITY | 2012 |
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| CODON-OPTIMIZED NUCLEIC ACID WHICH ENCODES SMN1 PROTEIN, AND USE THEREOF | 2020 |
|
RU2742837C1 |
| PRODUCTION OF AAV IN INSECT CELLS, METHODS AND COMPOSITIONS FOR THIS PURPOSE | 2017 |
|
RU2739596C2 |
| IMMUNISATION AGAINST CHLAMYDIA TRACHOMATIS | 2002 |
|
RU2331435C2 |
| PASSAGE VECTOR AND VACCINE FOR TUBERCULOSIS | 2007 |
|
RU2453603C2 |
| EXPRESSION VECTOR ELEMENT COMBINATION, NEW METHODS FOR OBTAINING PRODUCER CELLS AND THEIR APPLICATION FOR RECOMBINANT PRODUCTION OF POLYPEPTIDES | 2012 |
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RU2639519C2 |
