TEMPERATURICALLY INTELLIGENT MUTANT INTEIN FOR THE INSOLUBLE EXPRESSION OF THE PRECURSOR OF THE TARGET PROTEIN Russian patent published in 2017 - IPC C07K14/385 C12N15/70 C12R1/19 

FIELD: biotechnology.

SUBSTANCE: temperature-sensitive mutant PRP8 mini-intein from Penicillium chrysogenum insoluble for expression in Escherichia coli cells of the target protein precursor is provided. Mutant PRP8 mini-intein comprises a substitution of an amino acid residue leucine at position 93 to a proline residue, substitution of a cysteine residue at position 1 to an alanine residue and optionally contains a substitution of a cysteine residue at position 11 to tyrosine residue in the original sequence PRP8 mini-intein from Penicillium chrysogenum, consisting of 157 amino acid residues. When using a mutant intein combined and insoluble expression self-splitting ofpurified fusion protein from the refolding process that does not require use of proteases. The degree of processing for the modeling of SUMO target protein is more than 50%, and for neurotoxin CVIE - about 60%.

EFFECT: improved properties.

9 dwg, 8 ex