FIELD: biotechnology.
SUBSTANCE: method is developed to obtain in Saccharomyces cerevisiae yeast secreted fully functional phospholipase A2, including phospholipase A2 sequence of strain Streptomyces violaceoruber A-2688, including carrier mutation S31A, S82A and N108Q, and containing at N-end additional dipeptide, consisting of amino acid residues of serine and glycine. Proposed method involves biosynthesis of the precursor of the target protein, which is a version of phospholipase A2, containing an intein insertion between the glycine residue in position 75 and the serine residue in position 76. Invention also includes four precursor proteins which are encoded by sequences SEQ ID N: 1–4, respectively.
EFFECT: removing the intein insertion and converting the precursor protein into the active target protein by autocatalytic processing.
5 cl, 11 ex, 1 tbl, 2 dwg
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