FIELD: medicine.
SUBSTANCE: method of invention is based on double-stage multiplex PCR with production of fluorescently labeled fragments of DNA with further hybridisation of these fragments on microchip that contains set of specific discriminating oligonucleotides with a certain nucleotide sequence. Definition of tuberculosis mycobacteria resistance to rifampicin and isoniazid is carried out by detection of dot nucleotide substitutions in DNA of microorganism. Invention is also related to set of primers, biochip and set of oligonucleotide probes used in method realisation.
EFFECT: invention makes it possible to perform analysis directly from clinical sample, to simultaneously detect several mutations, to reduce prime cost of analysis and to reduce time of its performance.
9 cl, 6 dwg, 2 tbl, 10 ex
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Year |
Author |
Number |
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2000 |
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