DEVICE FOR CELL FRACTION ISOLATION FROM HUMAN AND ANIMAL TISSUES, AND METHOD OF ITS APPLICATION Russian patent published in 2017 - IPC C12M3/06 C12M1/12 C12N5/77 A61K35/12 A61P41/00 

FIELD: biotechnology.

SUBSTANCE: device is proposed for isolation of stromal-vascular fraction from human and animal tissues, a method of cellular fractions isolation using this device, stromal-vascular fraction of adipose tissue to create tissue-engineering structures and for use as a cell autograft, and a method of tissue treatment, repair or replacement using stromal-vascular fraction of adipose tissue. The device includes a sealed container for tissue processing with a system of channels and nozzles-adapters. Also, this container has a conical shape and is divided into two chambers by the microfilter system. One chamber serves for tissue treatment and the other for cells concentrating. The container also has channels within the wall, wherein one of the channel comes out into the first chamber and the other into the second chamber. The method comprises decanting, washing and lipoaspirate enzymatic treatment, centrifugation, filtration through microfilters, followed by removal of fat impurities and stromal tissue, as well as secondary washing and cell fraction concentration.

EFFECT: controlled isolation of stromal-vascular fraction of adipose tissue, characterized by high cell survival, lack of debris and residues of stromal and adipose tissue and circulating blood cells, and low concentration of residual enzyme activity.

8 cl, 7 dwg, 1 tbl