FIELD: medicine.
SUBSTANCE: method for adipose tissue preparation for cryopreservation includes lipoaspyrate washing, mixing of the washed fat tissue with a cryopreserving solution containing dimethylsulfoxide, human albumin and dextran, controlled freezing and storage in liquid nitrogen. In the cryopreservation solution, succinic acid is administered in terms of 3 mg/100 ml of adipose tissue.
EFFECT: method for adipose tissue preparation for cryopreservation allows to increase the viability of adipose tissue cellular fraction during its cryopreservation and thawing.
1 tbl, 1 ex
| Title | Year | Author | Number |
|---|---|---|---|
| METHOD FOR LIPOASPIRATE PROCESSING | 2016 |
|
RU2627606C1 |
| METHOD FOR REDUCING TOXICITY OF CRYOPRESERVATION SOLUTION BASED ON DIMETHYL SULFOXIDE AFTER THAWING OF HEMATOPOIETIC STEM CELLS | 2020 |
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