METHOD FOR ESTIMATION OF COW MILK PROTEIN OXIDATIVE MODIFICATION INDICATORS Russian patent published in 2017 - IPC G01N21/00 

FIELD: veterinary medicine.

SUBSTANCE: method for estimation of cows milk protein oxidative modification indicators involves determination of carbonylated proteins content, indicators evaluation is carried out by the carbonyl stress intensity factor, which is calculated by the formula: C_s=C_{KDNPH neutr} / C_{KDNPH base}, where C_{KDNPH neutr} is the content of ketone-dinitrophenylhydrazones of neutral character; C_{KDNPH base} is the content of ketone-dinitrophenylhydrazones of basic character, where C_s=1.5-1.9 indicates an early stage of carbonyl stress development in cows; C_s=2.0-3.4 indicates absence of carbonyl stress in clinically healthy cows; C_s=3.5-4.5 indicates a late stage of carbonyl stress development in cows. Calculation of the carbonyl stress intensity factor allows to reveal deep violations of the ratios of neutral and basic amino acid derivatives.

EFFECT: method is simple and economical in implementation, increased informative value of laboratory research.

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