METHOD OF DETERMINING OXIDATIVE MODIFICATION OF FIBRINOGEN IN BLOOD PLASMA BY CONTENT OF CARBONYL GROUPS IN FIBRINOUS CLOT Russian patent published in 2016 - IPC G01N33/68 

FIELD: medicine.

SUBSTANCE: invention refers to clinical biochemical laboratory diagnostics, namely to methods for determining modified proteins and is intended for selective quantitative determination of degree of oxidation of Fibrinogen in clinical samples of blood plasma by content of Carbonyl groups in fibrinous clot. Fibrinous clot is washed, dried, subjected to hydrolysis, hydrolysate is cooled, concentration of protein determined in it, and fibrinogen concentration is converted into mg/ml of plasma. For determination of carbonyl groups to 0.5 ml of hydrolysate 2 ml of 0.01 M solution of 2,4-dinitrophenylhydrazine in 2 M of HCl is added, incubated, added cooled 20 % solution of TCA in volume ratio to reaction mixture of 8:5, held at -20 °C, centrifuged; obtained precipitate is washed with triple centrifugation, successively using 5, 3 and 2 ml of ethanol and ethyl acetate mixture in ratio 1:1. After drying precipitate is dissolved in 2.5 ml of 8 M urea and centrifuged once again. Measured optical density of dinitrophenylhydrazines. Invention advantage is its analytical reliability, as well as simplicity and availability.

EFFECT: method is suitable for use in clinical diagnostic laboratory.

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