DETERMINATION OF OXIDATIVE MODIFICATION OF BLOOD PLASMA FIBRINOGEN Russian patent published in 2007 - IPC G01N33/86 

FIELD: clinical biochemistry, in particular evaluation of fibrinogen oxidative modification degree under oxidative stress action.

SUBSTANCE: claimed method includes treatment of tested sample with 20 % trichloroacetic acid (TCA) solution, addition of 1 ml of 0.1 M 2,4-dinitrophenyl hydrazine in 2 M HCl to precipitate; incubation at room temperature for 1 h; centrifugation at 3000 g for 20 min; three-stage precipitate washing with 1 ml 1:1 ethanol/acetate solution; precipitate drying; dissolving thereof in 2.5 ml of 8 M urea followed by evaluation of protein oxidative modification based on level of formed dinitrophenyl hydrazones using spectrophotometrical detection of optical density, wherein 0.5 ml of 20 mM CaCl₂ solution is added in 1 ml of citrate blood plasma, mixture is incubated on water bath at 37°C for 10 min, fibrin clot is isolated, dried on paper filter, weighted, converted to fibrinogen concentration (mg/ml), and before treatment with 20 % TCA solution 0.5 ml of 0.9 % NaCl solution is added to fibrinogen clot and further TCA solution is added in ratio of 1:1.

EFFECT: accelerated method of high information content.