FIELD: biotechnology.
SUBSTANCE: genetic DNA construct represented by the nucleotide sequence SEQ ID NO 2 containing a codon-optimized gene encoding the glycoprotein (G protein) of rabies virus with the consensus amino acid sequence SEQ ID NO 1, taking into account the variety of the rabies virus strains circulating in the territory of the Russian Federation is proposed. The proposed DNA construct in the pVAX1 vector in the transfected human cell culture, provides an increase in protein G gene expression as compared to the expression of the gene of the Vnukovo-32 strain viral protein G in a similar vector.
EFFECT: proposed DNA construct can be used to create an anti-rabies DNA vaccine that provides efficient glycoprotein synthesis in mammalian cells.
2 dwg
| Title | Year | Author | Number |
|---|---|---|---|
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|
RU2707544C1 |
| NUCLEOTIDE SEQUENCE OPTIMIZED FOR EXPRESSION IN BACTERIA OF CONSENSUS GLYCOPROTEIN OF RABIES VIRUS | 2018 |
|
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| TRIMERISED SINGLE-DOMAIN ANTIBODY THAT SPECIFICALLY BINDS WITH GLYCOPROTEIN G OF RABIES VIRUS, NEUTRALISING RABIES VIRUS | 2013 |
|
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| NEUTRALIZING MONOCLONAL ANTIBODY BINDING TO RABIES VIRUS GLYCOPROTEIN G, DNA FRAGMENTS CODING SAID ANTIBODY, AND ANTIGEN-BINDING FRAGMENT | 2017 |
|
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| RECOMBINANT FUSION PROTEIN SPECIFICALLY BINDING AND NEUTRALIZING RABIES VIRUS, METHOD FOR PRODUCTION AND USE THEREOF | 2023 |
|
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| FRAGMENT OF DNA, CODING SYNTHESIS OF GLYCOPROTEIN OF RABIES G VIRUS, RECOMBINANT PLASMIDAL DNA PVG18-1, CODING GLYCOPROTEIN OF RABIES G VIRUS, ISOLATE OF BACTERIUM EXCHERICHIA COLI IS PRODUCENT OF GLYCOPROTEIN OF RABIES G VIRUS | 1991 |
|
RU2008355C1 |
| SET OF SYNTHETIC OLIGONUCLEOTIDE PRIMERS FOR IDENTIFICATION OF RNA OF RABIES VIRUS AND METHOD OF IDENTIFICATION OF RNA OF RABIES VIRUS BY MEANS OF SYNTHETIC OLIGONUCLEOTIDE PRIMERS IN POLYMERASE CHAIN REACTION WITH REVERSE TRANSCRIPTION (RT-PCR) | 2014 |
|
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| HUMANISED ANTIGEN-BINDING FRAGMENTS (Fab) AGAINST RABIES VIRUS, ISOLATED DNA FRAGMENT, CODING Fab AGAINST RABIES VIRUS, YEAST CELL, TRANSFORMED BY DNA FRAGMENT, AND METHOD OF OBTAINING Fab AGAINST RABIES VIRUS WITH APPLICATION OF YEASTS | 2010 |
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RU2511440C2 |
