FIELD: biotechnology.
SUBSTANCE: invention provides a method for bacillus thuringiensis strains genetic passportization by means of multiplex express analysis of reference DNA sequences. Bacterial DNA is isolated, multilocus PCR is performed to prepare DNA reference complexes using primers containing the sequences of SEQ ID NO: 1-60 and combinations thereof, pyrosequencing the resulting DNA reference complexes to obtain unique genetic certificates of Bacillus thuringiensis strains sufficient to separate strains within one biovar.
EFFECT: invention is a high-performance express method that allows determination of Bacillus thuringiensis bacterium specific strains within a single biovar, its application leads to a reduction in the analysis cost and a reduction in its implementation timing.
13 cl, 1 tbl, 1 ex
| Title |
Year |
Author |
Number |
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|
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| INSECTICIDAL PROTEINS EXTRACTED FROM Bacillus BACTERIA AND USE THEREOF |
2004 |
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- Pitkin Dzhon V.
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|
RU2382822C2 |
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2019 |
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- Zakharova Irina Borisovna
|
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2023 |
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|
RU2822737C1 |
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2007 |
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| SET OF REAGENTS AND METHOD FOR DETECTING DNA OF CAUSATIVE AGENTS OF PLAGUE, ANTHRAX AND TULAREMIA BY PCR METHOD WITH HYBRIDISATION-FLUORESCENT RECORDING OF RESULTS |
2013 |
- Mokrievich Aleksandr Nikolaevich
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- Varlamov Dmitrij Aleksandrovich
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|
RU2542395C1 |
| RECOMBINANT POLYNUCLEOTIDE SEQUENCE MARKING UNIQUE TRANSFORMATION ACTION BETWEEN GENETIC MAKER INCLUDING GENE cryIIIA AND GENOMIC DNA OF LUGOVSKOY POTATO; ITS APPLICATION; CELL, TRANSGENIC PLANT, AND ITS PROGENY CONTAINING THIS SEQUENCE |
2007 |
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- Kuznetsov Boris Borisovich
- Skrjabin Konstantin Georgievich
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|
RU2337529C1 |
| METHOD FOR MOLECULAR LABELLING, BASED ON MICROSATELLITE LOCI, INTENDED FOR GENETIC CERTIFICATION OF PLANT VARIETY OF RUBUS GENUS PLANTS |
2018 |
- Vidyagina Elena Olegovna
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|
RU2732922C2 |
| RECOMBINANT POLYNUCLEOTIDE SEQUENCE CHARACTERIZING UNIQUE TRANSFORMATION ACT BETWEEN GENETIC CONSTRUCT INCLUDING cryIIIa GENE, AND GENOMIC DNA OF GENUS ELIZAVETA POTATO, USES THEREOF, TRANSGENIC PLANT AND GENERATION THEREOF |
2004 |
- Kamionskaja Anastasija Mikhajlovna
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- Skrjabin Konstantin Georgievich
|
RU2286385C2 |
| RECOMBINANT POLYNUCLEOTIDE SEQUENCE CHARACTERIZING UNIQUE TRANSFORMATION ACT BETWEEN GENETIC CONSTRUCT INCLUDING cryIIIa GENE, AND GENOMIC DNA OF GENUS NEVSKIY POTATO, USES THEREOF, TRANSGENIC PLANT AND GENERATION THEREOF |
2005 |
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- Kuznetsov Boris Borisovich
- Skrjabin Konstantin Georgievich
|
RU2286386C1 |
