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2 627 187

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IPC

C12N1/20(2006-01-01)

C12P7/40(2006-01-01)

C12R1/07(2006-01-01)

(21) (22)

Application

2016129966, 2016-07-21

(24)

Start date

2016-07-21

(22)

Actual filing date

2016-07-21

(45)

Published

2017-08-03

(72)

Inventor

Efremenkova Olga VladimirovnaMalanicheva Irina AlekseevnaZenkova Valentina AleksandrovnaMalyutina Natalya MikhajlovnaVasileva Byazilya FejzullovnaSumarukova Irina GeorgievnaReznikova Marina IlinichnaBajshev Iosif TagirovichEfimenko Tatyana AleksandrovnaRogozhin Evgenij AleksandrovichSalimova Elena IsaevnaKorolev Aleksandr MikhajlovichOsterman Ilya AndreevichSergiev Petr Vladimirovich

(73)

Holder

Federalnoe Gosudarstvennoe Byudzhetnoe Nauchnoe Uchrezhdenie Institut Po Izyskaniyu Novykh Antibiotikov Imeni G.F. Gauze"

BACILLUS PUMILUS STRAIN AND METHOD FOR OBTAINING AMIKUMACIN A ANTIBIOTIC WITH ITS APPLICATION Russian patent published in 2017 - IPC C12N1/20 C12P7/40 C12R1/07

Abstract RU 2627187 C1

FIELD: biotechnology.

SUBSTANCE: method for amikumacin obtaining involves cultivation of a Bacillus pumilus bacteria strain VKPM B-12548 on a liquid nutrient medium containing sucrose, glucose, yeast extract, K₂HPO₄, NaCl, MgSO₄, (NH₄)₂SO₄, FeSO₄×7H₂O, MnCl₂×4H₂O, CaCO₃ and tap water at a predetermined ratio of components, followed by cultivation for 40-42 hours under aerating conditions. Cells from the culture liquid are precipitated by centrifugation to obtain a supernatant. Amberlite XAD-2 sorbent is added to the supernatant, followed by elution of amikumacin A with n-butanol-acetone-water system at a ratio of 1:1:1.

EFFECT: invention allows to increase the yield of amikumacin A antibiotic.

2 cl, 3 dwg, 1 tbl, 1 ex

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RU 2 627 187 C1

Authors

Efremenkova Olga Vladimirovna

Malanicheva Irina Alekseevna

Zenkova Valentina Aleksandrovna

Malyutina Natalya Mikhajlovna

Vasileva Byazilya Fejzullovna

Sumarukova Irina Georgievna

Reznikova Marina Ilinichna

Bajshev Iosif Tagirovich

Efimenko Tatyana Aleksandrovna

Rogozhin Evgenij Aleksandrovich

Salimova Elena Isaevna

Korolev Aleksandr Mikhajlovich

Osterman Ilya Andreevich

Sergiev Petr Vladimirovich

Dates

2017-08-03—Published

2016-07-21—Filed