FIELD: biotechnology.
SUBSTANCE: group of inventions is described including a set of oligonucleotide primers and fluorescence-labelled probes for identification of colon and rectum malignant lesions by analyzing RCGY sites methylation in the DNA samples in the specified positions of the regulatory regions of the tumor marker genes by the GLAD-PCR method and a method for identification of colon and rectum malignant lesion by RCGY sites methylation analysis in DNA samples of in the specified positions of regulatory regions of tumor marker genes by the GLAD-PCR method. This method involves formation of a colorectal cancer tumor marker gene panel: ADHFE1, ALX4, CNRIP1, EID3, ELMO1, ESR1, FBN1, HLTF, LAMA1, NEUROG1, NGFR, RARB, RXRG, RYR2, SDC2, SEPT9, SFRP2, SOCS3, SOX17, THBD , TMEFF2, UCHL1 and VIM.
EFFECT: invention expands the arsenal of oligonucleotide primers and fluorescence-labelled primers.
2 cl, 3 dwg, 4 tbl, 3 ex, 1 app
| Title | Year | Author | Number |
|---|---|---|---|
| METHOD OF DETERMINING THE METHYLATION SITES PuCGPy REGULATORY REGIONS OF GENES-MARKERS OF COLORECTAL CANCER BY GLAD-PCR-ANALYSIS AND OLIGONUCLEOTIDE PRIMERS AND FLUORESCENT-LABELLED PROBES FOR REALISING SAID METHOD | 2015 |
|
RU2596404C1 |
| SET OF OLIGONUCLEOTIDE PRIMERS AND FLUORESCENTLY LABELLED PROBES FOR IDENTIFYING THE EPIGENETIC MARKERS OF CERVICAL CANCER | 2021 |
|
RU2760573C1 |
| METHOD FOR DETERMINING HUMAN AGE BY DNA | 2021 |
|
RU2797018C1 |
| METHOD OF DETERMINING NUCLEOTIDE SEQUENCE Pu(5mC)GPy AT PREDETERMINED POSITION OF LONG-DISTANCE DNA | 2013 |
|
RU2525710C1 |
| METHOD FOR DETECTION AND ANALYSIS OF METHYLATION OF GENOMIC DNA REGIONS IN BIOLOGICAL SAMPLES OF PERIPHERAL BLOOD OF PATIENTS WITH NON-HODGKIN'S LYMPHOMA | 2022 |
|
RU2804962C1 |
| METHOD OF DETERMINING NUCLEOTIDE SEQUENCE R(5mC)GY IN PRESET POSITION OF EXTENDED DNA | 2015 |
|
RU2587631C1 |
| METHOD OF DETERMINING HYPERMETHYLATED CpG ISLANDS IN REGION OF SUPPRESSOR GENES OF TUMOUR GROWTH IN HUMAN DNA | 2009 |
|
RU2413773C1 |
| METHOD OF MAPPING POSITIONS OF ROW OF METHYLATED NUCLEOTIDE SEQUENCES Pu(5mC)GPy IN EXTENDED DNA FOR CONSTRUCTING EPIGENETIC PROFILE AND DETECTION OF ABNORMAL METHYLATED DNA SECTIONS | 2015 |
|
RU2586502C1 |
| PRIMER KIT, REAGENT AND COMMERCIAL KIT FOR METHYLATION OF SPECIFIC GENE REGIONS ASSOCIATED WITH HUMAN COLORECTAL CANCER AND USE OF THE COMMERCIAL KIT | 2019 |
|
RU2791172C1 |
| EARLY DNA-DIAGNOSTIC TECHNIQUE OF PROSTATE CANCER | 2009 |
|
RU2405837C1 |
