FIELD: medicine.
SUBSTANCE: claimed is method of determining hypermethylated CpG is the region of suppressor genes of tumour growth in human DNA, which includes obtaining of samples of highly purified DNA; fragmentation of isolated DNA with restriction endonuclease which does not have a recognition site in amplified region, in particular TaqI; hydrolysis of fragmented DNA with methyl-dependent site-specific endonucleases GlaL and Blsl, carried out in two separate samples; further amplification of hydrolysis products with application of a pair of primers, flanking gene area which contains CpG islands and making a conclusion on the basis of changes in comparison with control, determined in at least one of the two test samples during their electrophoretic separation.
EFFECT: invention method ensures possibility of efficient diagnostics of cancer diseases.
2 cl, 3 dwg, 3 ex
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