FIELD: biotechnology.
SUBSTANCE: method has been proposed for detecting and analysing methylation of genomic DNA regions in biological samples of peripheral blood from patients with non-Hodgkin's lymphoma. The method involves digesting a sample of genomic DNA using a methyl-dependent endonuclease to produce fragments. It also involves sequencing fragments to obtain reads, aligning the reads to a reference sequence to select a uniquely aligned read, identifying the site in the reference sequence that is methylated in the uniquely aligned read. GlaI is used as a methyl-dependent endonuclease, which is used to analyse the Pu(5mC)GPy sequence in at least one of the genes.
EFFECT: technical result is to increase the reliability and reliability of the method for detecting and analysing methylation of genomic DNA regions through a more reliable and accurate determination of the positions (mapping) of a large number of methylated sites in the genome, represented by the sequence Pu(5mC)GPy.
1 cl, 1 tbl
| Title | Year | Author | Number |
|---|---|---|---|
| METHOD OF MAPPING POSITIONS OF ROW OF METHYLATED NUCLEOTIDE SEQUENCES Pu(5mC)GPy IN EXTENDED DNA FOR CONSTRUCTING EPIGENETIC PROFILE AND DETECTION OF ABNORMAL METHYLATED DNA SECTIONS | 2015 |
|
RU2586502C1 |
| METHOD OF DETERMINING NUCLEOTIDE SEQUENCE Pu(5mC)GPy AT PREDETERMINED POSITION OF LONG-DISTANCE DNA | 2013 |
|
RU2525710C1 |
| METHOD OF DETERMINING THE METHYLATION SITES PuCGPy REGULATORY REGIONS OF GENES-MARKERS OF COLORECTAL CANCER BY GLAD-PCR-ANALYSIS AND OLIGONUCLEOTIDE PRIMERS AND FLUORESCENT-LABELLED PROBES FOR REALISING SAID METHOD | 2015 |
|
RU2596404C1 |
| METHOD OF DETERMINING NUCLEOTIDE SEQUENCE R(5mC)GY IN PRESET POSITION OF EXTENDED DNA | 2015 |
|
RU2587631C1 |
| METHOD FOR DETERMINATION OF METHYLATION OF COLORECTAL CANCER TUMOR MARKER GENES REGULATORY REGIONS PUCGPY SITES BY GLAD-PCR ANALYSIS, AND SET OF OLIGONUCLEOTIDE PRIMERS AND FLUORESCENCE-LABELLED PROBES FOR IMPLEMENTATION OF THIS METHOD | 2016 |
|
RU2630669C1 |
| METHOD OF DETERMINING HYPERMETHYLATED CpG ISLANDS IN REGION OF SUPPRESSOR GENES OF TUMOUR GROWTH IN HUMAN DNA | 2009 |
|
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| SET OF OLIGONUCLEOTIDE PRIMERS AND FLUORESCENTLY LABELLED PROBES FOR IDENTIFYING THE EPIGENETIC MARKERS OF CERVICAL CANCER | 2021 |
|
RU2760573C1 |
| SET OF SYNTHETIC OLIGONUCLEOTIDES FOR METHYL-SENSITIVE DNA AMPLIFICATION | 2017 |
|
RU2662664C1 |
| STRAIN OF BACTERIA MICROCOCCUS LUTEUS 805 - PRODUCER OF SITE-SPECIFIC METHYL-DEPENDEDN ENDONUCLEASE MluVI | 2016 |
|
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|
RU2593723C1 |
