FIELD: biotechnology.
SUBSTANCE: invention relates to a method for determining the probable age of an unknown individual. The essence of the method is to analyze the level of methylation of epigenetic age markers — RCGY sites located in the regions of genes whose methylation is associated with age, using GLAD-PCR analysis and calculate the age value based on the obtained values.
EFFECT: development of a method for simplifying the procedure and reducing the analysis time, as well as using a small amount of DNA for analysis, increasing the reproducibility of the result and, as a result, the reliability of age determination.
1 cl, 1 tbl, 1 ex
| Title | Year | Author | Number |
|---|---|---|---|
| METHOD OF DETERMINING THE METHYLATION SITES PuCGPy REGULATORY REGIONS OF GENES-MARKERS OF COLORECTAL CANCER BY GLAD-PCR-ANALYSIS AND OLIGONUCLEOTIDE PRIMERS AND FLUORESCENT-LABELLED PROBES FOR REALISING SAID METHOD | 2015 |
|
RU2596404C1 |
| METHOD FOR DETERMINATION OF METHYLATION OF COLORECTAL CANCER TUMOR MARKER GENES REGULATORY REGIONS PUCGPY SITES BY GLAD-PCR ANALYSIS, AND SET OF OLIGONUCLEOTIDE PRIMERS AND FLUORESCENCE-LABELLED PROBES FOR IMPLEMENTATION OF THIS METHOD | 2016 |
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| EARLY DNA-DIAGNOSTIC TECHNIQUE OF PROSTATE CANCER | 2009 |
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| METHODS FOR DNA AMPLIFICATION TO PRESERVE METHYLATION STATUS | 2018 |
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| METHOD FOR FORMATION OF DNA METHYLATION MARKER SYSTEMS | 2011 |
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| DIAGNOSTIC MARKERS | 2012 |
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RU2614254C2 |
| SET OF OLIGONUCLEOTIDE PRIMERS AND PROBES AND METHOD FOR QUANTITATIVE DETERMINATION OF FETAL DNA IN BLOOD OF PREGNANT WOMAN BASED ON ANALYSIS OF HYPERMETHYLATED FETAL DNA SECTIONS | 2016 |
|
RU2642622C2 |
