METHOD FOR DETECTING LUJO VIRUS RNA BY REVERSE TRANSCRIPTION AND POLYMERASE CHAIN REACTION, TAKING INTO ACCOUNT REAL-TIME RESULTS Russian patent published in 2021 - IPC C12N15/00 

FIELD: medicine.

SUBSTANCE: invention relates to medicine, in particular to medical diagnostics, specifically to a method for determining the RNA of the Lujo virus (a member of the family Arenaviridae) with the use of reverse transcription and a real-time polymerase chain reaction (RT-PCR) on the basis of biological material of people, animals and environmental objects and can be used in research and medical institutions, agencies of Rospotrebnadzor. The accumulation of the fluorescent signal along the JOE channel in the samples under study indicates the presence of the RNA of Lujo and the result is considered valid if the accumulation of the fluorescent signal of an internal control sample is observed in the same samples via the FAM channel.

EFFECT: invention constitutes a method for detecting the RNA of the Lujo virus which involves carrying out reverse transcription and PCR taking into account the results in real time using the appropriate primers and probes. After that one can detect the RNA of Lujo on the basis of the presence and absence of intersection of the fluorescence curve in each of the channels used with the threshold line set at the appropriate level.

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