METHOD FOR SAMPLE PREPARATION OF HEPATITIS B VIRUS DNA SAMPLES FOR WHOLE GENOME SEQUENCING AND OLIGONUCLEOTIDE PRIMERS FOR ITS IMPLEMENTATION Russian patent published in 2024 - IPC C12Q1/70 

FIELD: biotechnology; medicine; molecular biology.

SUBSTANCE: disclosed is a method for preparing DNA samples, comprising steps of: (a) isolating DNA from a biological material; (b) performing PCR using two pools of oligonucleotide primers, where for the first pool oligonucleotide primers having the structure SEQ ID NO: 1–30, and for the second pool oligonucleotide primers having the structure SEQ ID NO: 31–55; (c) purifying PCR products using magnetic particles; (d) indexing the obtained amplicons using primers containing adapter sequences SEQ ID NO: 56, 57; (e) repeated purification of PCR products using magnetic particles; (f) evaluation of PCR products obtained during amplification by means of electrophoresis. Disclosed is a pool of oligonucleotide primers for implementing the method, having the structure SEQ ID NO: 1–57, to perform the function of primers complementary to hepatitis B virus gene regions.

EFFECT: invention enables to perform sample preparation of libraries containing target fragments of hepatitis B virus (HBV) genes, by high-throughput sequencing for the purpose of searching for hepatitis B virus mutations, without reducing quality and volume of performed studies.

10 cl, 9 tbl, 4 ex