FIELD: biotechnology.
SUBSTANCE: method comprises culturing of a prokaryotic host cell producing a recombinant protein, isolation of the said recombinant protein, and purification to a filtered storage preparation (FBS). The step of isolation of the said recombinant protein comprises homogenization. This method is characterized by the level of dissolved oxygen in recovery of the said recombinant protein being maintained at 75% or more before homogenization and at 50% or more after homogenization. The invention also relates to a method for producition of a recombinant protein comprising prokaryotic host cell culturing with deletion of the menE gene. The method comprises culturing of the said prokaryotic host cell producing a recombinant protein, recombinant protein isolation and purification to FBS. The methods of the invention are characterized by the obtained FBS containing no detectable amounts of the recombinant protein adduct and 1,4-dihydroxy-2-naphthoate (DHNA) according to the data of ion-exchange chromatography (IEC) at 310 nm.
EFFECT: invention allows to obtain a recombinant protein, the filtered preparation of which does not contain brown adduct and meets technical requirements.
18 cl, 12 dwg, 3 tbl, 5 ex
