FIELD: medicine.
SUBSTANCE: invention is intended to determine the share of fetal DNA in the plasma of a pregnant woman using high-productive sequencing methods. 53 single nucleotide polymorphisms (SNP) located on 1-12 chromosomes with a heterozygote frequency of 40-50%, and specific primers to them are used. The share of fetal DNA is determined as a median of the fetal DNA share value for all informative SNPs.
EFFECT: effective determination of the share of fetal DNA in the blood plasma of a pregnant woman, regardless of the sex of the fetus.
4 tbl, 2 ex
| Title | Year | Author | Number |
|---|---|---|---|
| NONINVASIVE DIAGNOSTIC DNA TEST FOR ANEUPLOIDY DETECTION | 2012 |
|
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| METHOD OF DETERMINING THE SOURCE OF ANEUPLOID CELLS ON THE BLOOD OF A PREGNANT WOMAN | 2016 |
|
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| METHOD FOR DETERMINING FOETAL KARYOTYPE OF PREGNANT WOMAN BASED ON SEQUENCING HYBRID READINGS CONSISTING OF SHORT FRAGMENTS OF EXTRACELLULAR DNA | 2019 |
|
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| METHODS FOR NON-INVASIVE PRENATAL PLOIDY CALLING | 2011 |
|
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| HIGHLY MULTIPLEX PCR METHODS AND COMPOSITIONS | 2012 |
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| METHODS OF NONINVASIVE PRENATAL PATERNITY DETERMINATION | 2011 |
|
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| METHODS OF DETECTING AND MONITORING CANCER BY PERSONALIZED DETECTION OF CIRCULATING TUMOR DNA | 2019 |
|
RU2811503C2 |
| METHOD FOR NON-INVASIVE PRENATAL SCREENING OF FETAL ANEUPLOIDY | 2019 |
|
RU2712175C1 |
| NON-INVASIVE DIAGNOSTIC TECHNIQUE FOR FOETAL ANEUPLOIDY BY SEQUENCE ANALYSIS | 2014 |
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RU2543155C1 |
| DETECTION OF MUTATIONS AND PLOIDY IN CHROMOSOMAL SEGMENTS | 2015 |
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RU2717641C2 |
