FIELD: medicine.
SUBSTANCE: blood sampling, leukocytes selection, incubation, fixation and cells staining are performed. Adhesiveness of cells due to their aggregability among themselves, location and composition of the interacting cells is also taken into account. Further, the leukocytes viability is determined. To do this, the cells are incubated with and without phytohemagglutinin. Then, the number of aggregated cells forming cell aggregation agglomerates is counted in the preparations. Leukocyte indices of mitogen-induced adhesiveness are determined as percentage and quantitative values of leukocyte mitogen-induced adhesiveness indexes in patients are compared to the reference values of these indices in almost healthy individuals.
EFFECT: invention allows to evaluate the mitogen-induced adhesiveness of leukocytes in patients.
1 tbl, 2 ex
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Authors
Dates
2017-12-05—Published
2016-06-14—Filed