FIELD: biotechnology.
SUBSTANCE: invention relates to glutamine-free cell culture media supplemented with asparagine and can be used to prepare a polypeptide selected from antibodies, antibody fragments and immunoadhesins in a mammalian host cell. To prepare the polypeptide in the production phase, a ready-to-use cell culture medium without glutamine is provided which contains asparagine 7.5 mM to 15 mM and aspartic acid 1 mM to 10 mM.
EFFECT: invention allows to increase the cell culture lifespan, specific productivity and the final titer of the recombinant protein.
42 cl, 13 dwg, 1 ex
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