FIELD: biotechnology.
SUBSTANCE: nucleic acid molecule encodes a polypeptide consisting of two methionine residues as the first and second N-terminal amino acid residues linked through a peptide bond to the mature eukaryotic histone H1. 3. A polypeptide is prepared by culturing a host cell transformed with an expression vector comprising the said nucleic acid molecule. The polypeptide is used as part of a pharmaceutical composition for treatment of cancer, bacterial, viral or fungal infections. Also, the polypeptide is used as part of a composition for diagnosing a patient with respect to the presence of a response to a pharmaceutical composition containing the said polypeptide, or with respect to curability thereof.
EFFECT: invention allows to increase the efficiency of recombinant expression and facilitate the determination of the said polypeptide in the presence of endogenous histones while maintaining biological activity of the mature eukaryotic histone.
16 cl, 3 dwg, 6 tbl, 7 ex
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Year |
Author |
Number |
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|
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|
RU2669937C1 |
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2002 |
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|
RU2297425C2 |
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2006 |
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|
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2004 |
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|
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2005 |
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2011 |
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|
RU2491342C1 |
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2011 |
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|
RU2458129C1 |
