FIELD: biotechnology.
SUBSTANCE: method comprises introduction of an expression construct into a microorganism that contains a promoter and a nucleic acid encoding a protein and protein expression in a microorganism. This method is characterized by the microorganism belonging to the Bacillus pumilus species and its spore formation is inhibited by the deletion of the spoIV gene or parts thereof.
EFFECT: method allows to obtain the target protein in high yield.
2 cl, 6 tbl, 4 ex
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| EXPRESSION METHOD | 2012 |
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RU2642324C2 |
| EXPRESSION VECTORS FOR IMPROVED PROTEIN SECRETION | 2012 |
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RU2661790C2 |
| IMPROVED PRODUCTION OF PROTEIN IN BACILLUS | 2008 |
|
RU2515112C2 |
| POLYPEPTIDES HAVING ENDOPEPTIDASE ACTIVITY AND POLYNUCLEOTIDES CODING SAME | 2011 |
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| ENZYME PROTEASE AND ITS APPLICATION | 2012 |
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RU2611043C2 |
| OBTAINING 1,4-DIAMINOBUTANE | 2010 |
|
RU2577967C2 |
| METHODS FOR APPLICATION OF THERMOSTABLE SERINE PROTEASES | 2017 |
|
RU2771261C2 |
| METHODS FOR ENHANCING THE DEGRADATION OR CONVERSION OF CELLULOSIC MATERIAL | 2006 |
|
RU2441912C2 |
| TANDEM PROMOTER FUNCTIONING IN BACTERIA OF BACILLUS GENUS, TRANSFORMED BACILLUS BACTERIUM - TARGET PRODUCT PRODUCER CONTAINING SAID PROMOTER, AND METHOD FOR PRODUCING DESIRED PRODUCT USING SAID BACTERIUM | 2019 |
|
RU2723722C1 |
| BACTEROICIN AGAINST LYSTERIA | 2001 |
|
RU2250267C2 |
