FIELD: biotechnology.
SUBSTANCE: immunochromatographic test system for detection of pathogenic H. pylori strains based on cagA protein is a multimembrane composite consisting of a nitrocellulose membrane with a membrane impregnated with a monoclonal antibody conjugate, a HP-387 clone at a concentration of 20 mcg⋅cm-3 with nano-particles of colloidal gold with an average diameter of 30 nm, a sample membrane and an adsorbing membrane. Monoclonal antibodies, HP-1811 clone at a concentration of 12 mcg⋅cm-3 are applied to the analytical membrane in the test zone, and in the control zone - goat anti-species antibodies against mouse Ig are applied at a concentration of 10 mcg⋅cm-3.
EFFECT: invention provides determination of the said protein in various biological materials.
1 dwg, 1 tbl, 3 ex
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Authors
Dates
2018-01-25—Published
2017-03-14—Filed