METHOD OF IMMUNOCHROMATOGRAPHIC ASSAY OF MILK AND DAIRY PRODUCTS FOR ANTIBIOTICS Russian patent published in 2010 - IPC G01N33/50 A23C9/158 

FIELD: medicine.

SUBSTANCE: there is used a multi-membrane composite in certain regions of which there are applied: 1) coloured marker particles in complex with antibiotic specific antibodies 1, 2) antibiotic conjugate with a carrier molecule providing conjugate immobilisation on the membrane, 3) membrane immobilised antibodies 1 specific antibodies 2 (a reference zone). Once the multi-membrane composite contacts with sampled milk or dairy product to be tested, a coloured strip or strips are formed in a certain zone or zones of the multi-membrane composite to be interpreted as follows: a) the reference zone coloured - the presence of the antibiotics to be detected in the sample, b) the analytical and reference zones coloured - the absence of the antibiotics to be detected in the sample. A characteristic property of the offered approach is the use of a specially specified combination of antibodies 1 of high reaction rate with an antigen at a kinetic association reaction constant not less than 10⁵ M^-1 sec^-1, the membrane carriers of pore size not less than 10 mcm and coloured marker particles of average diametre not less than 30 nm.

EFFECT: invention allows non-laboratory express assay of dairy products for antibiotics.

4 dwg, 4 ex