METHOD OF CALCULATION OF FORECAST FOR EARLY DEVELOPMENT OF SKIN MELANOMA Russian patent published in 2018 - IPC G01N33/53 

FIELD: medicine.

SUBSTANCE: invention refers to medicine, namely to oncology, and can be used in order to predict the early progression of skin melanoma. Method of the invention comprises determination, among homogenates, the samples of tumor tissue and of the visually unchanged tissue that is adjacent to it (the peritumoral zone), which are taken during surgical treatment for skin melanoma, by the method of immunoenzymatic analysis of levels of IL-6 cytokines in tumor tissue, IL-2 and IL-1β in the tissue of the peritumoral zone. DNA is analyzed in skin melanoma tissue using the flow cytometry method. Clinical assessment of the prevalence of the disease is carried out, then the individual risk of progression of skin melanoma according is calculated according to the formula

,

where K is the forecast coefficient, z is the linear regression coefficient, which is calculated according to the formula z = (-4.1+0.065⋅X1+0.037⋅X2+1.06⋅X3+3.81⋅P-5.56⋅A), where X1 is the content of IL-6 in the tumor tissue (pg/ml/g), X2 is the content of IL-1 β in tissue IZ (pg/ml/g), X3 is the content of IL-2 in tissue IZ (pg/ml/g), while the nominal values are ranked as follows: prevalence of the tumor process (P): 0 – local, 1 – common, 2 – generalized (G), as well as the presence of the aneuploid or diploid cell clone (A): 1 – aneuploid clone, 0 – diploid clone, at the K value that is equal to or greater than 0.64, the high risk of early progression of skin melanoma is predicted, at the K value that is less than 0.64 – the low risk of early development of skin melanoma is predicted.

EFFECT: use of the invention allows to calculate the risk of early progression of skin melanoma.

1 cl, 2 ex, 7 tbl