METHOD FOR ONCOLOGICAL DISEASES COURSE PREDICTION Russian patent published in 2017 - IPC G01N33/53 

FIELD: medicine.

SUBSTANCE: in the patient's peripheral blood, the number of CD3⁺ T-lymphocytes is determined, 10*9/l, and their subpopulations: CD4⁺ and CD8⁺ lymphocytes, 10*9/l,%, in addition, the value of the quotient from dividing the number of CD4⁺ lymphocytes subpopulations by the number of CD8⁺ is calculated. After completion of the primary treatment course in the patient's peripheral blood, the absolute number of CD3⁺ T-lymphocytes, 10*9/l, spontaneously and stimulatively synthesizing proinflammatory cytokines, tumour necrosis factor (TNF-α), interleukin - 2 (IL-2), interferon γ (INF-γ). After that, the reactivity of the effect of each type of cytokine on the tumour process course is estimated by calculating the reaction coefficient for each of them according to the formula: Kp=nCD3⁺_stim/nCD3⁺_spont, where Kp is the response coefficient of the effect on the tumour process course for the corresponding pro-inflammatory cytokine, namely: TNF-α, INF-γ, IL-2; nCD3⁺_stim is the number of T-lymphocyte cells (CD3⁺) in the peripheral blood, synthesizing the corresponding cytokine in the test system after stimulation; CD3⁺_spont is the number of cells of CD3⁺ T-lymphocytes in the peripheral blood, synthesizing the corresponding cytokine spontaneously. The value of the quotient of division of the number of CD4⁺ lymphocytes subpopulations by the CD8⁺ number, and at the quotient value equal to 1 and above, at the reaction coefficient value in the given limits for at least two cytokines, namely: in the range from 80 to 120 for TNF-α, from 80 to 120 for IL-2, from 150 to 250 for INF-γ, disease development is predicted as normoreactive, the prognosis is favourable; at the value of the quotient of division of the number of CD4+ lymphocytes subpopulations by the CD8+ number less than 1, at the reaction coefficient for at least two cytokines above the upper limit, namely: above 120 for TNF-α and for IL-2, above 250 for INF-γ, disease development is predicted as hyper-reactive, the prognosis is favourable; at the reaction coefficient for at least two cytokines below the lower limit, namely: below 80 for TNF- and for IL-2, below 150 for INF-, disease development is predicted as hyporeactive, the prognosis is unfavourable; at the value of the quotient of division of the number of CD4⁺ lymphocytes subpopulations by the CD8⁺ number more than 1, at the reaction coefficient for at least two cytokines above the upper limit, namely: above 120 for TNF-α and for IL-2, above 250 for INF-γ, disease development is predicted as hyper-reactive, the prognosis is unfavourable; at the reaction coefficient for at least two cytokines below the lower limit, namely: below 80 for TNF-α and for IL-2, below 150 for INF-γ, disease development is predicted as hyporeactive, the prognosis is favourable.

EFFECT: application of this method allows to predict the course of oncological diseases by evaluating the relationship between the ratio of subpopulations of lymphocytes and TNF-α, IL-2 and INF-γ cytokines effect on the tumour process course.

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