FIELD: biotechnologies.
SUBSTANCE: invention refers to the field of biotechnology, specifically to the method for genetical typing of strains of the plague microbe (Yersinia pestis) and of the pseudotuberculous microbe (Yersinia pseudotuberculosis) using the set of synthetic oligonucleotide primers and fragment analysis of fluorescently labeled DNA amplicons at the capillary DNA analyzer.
EFFECT: set of 13 pairs of primers, in contrast to the existing analogues, allows to carry out multiplex PCR in one reaction mixture.
1 cl, 1 dwg, 1 tbl
| Title | Year | Author | Number |
|---|---|---|---|
| METHOD FOR IDENTIFYING YERSINIA PESTIS AND YERSINIA PSEUDOTUBERCULOSIS AND SIMULTANEOUS DIFFERENTIATION OF YERSINIA PESTIS OF MAIN AND CENTRAL ASIAN SUBSPECIES BY MULTIPLEX PCR | 2020 |
|
RU2737775C1 |
| METHOD FOR DIFFERENTIATING YERSINIA PESTIS STRAINS OF VARIOUS SUBSPECIES AND BIOVARS BY POLYMERASE CHAIN REACTION AND MULTILOCUS SEQUENCE TYPING | 2011 |
|
RU2471872C1 |
| METHOD FOR DIFFERENTIATION OF YERSINIA PESTIS STRAINS BY MOLECULAR GENETIC TYPING USING IS ELEMENTS AS GENETIC MARKERS | 2021 |
|
RU2767371C1 |
| METHOD FOR INDICATION AND IDENTIFICATION OF STRAINS OF THE PLAGUE PATHOGEN BY THEIR BELONGING TO THE SPECIES YERSINIA PESTIS, TO SUBSPECIES, BIOVARS, PHYLOGENETIC BRANCHES AND BY THE PRESENCE OF GENES OF PATHOGENICITY MAIN FACTORS BY THE DNA-CHIP METHOD | 2020 |
|
RU2734636C1 |
| METHOD FOR GENETIC DIFFERENTIATION OF YERSINIA PSEUDOTUBERCULOSIS STRAINS BY MOLECULAR-GENETIC TYPING | 2019 |
|
RU2736649C1 |
| METHOD OF DIFFERENTIATING YERSINIA PESTIS STRAINS ON BASIC AND NONBASIC SUBTYPES BY PCR METHOD IN REAL TIME MODE | 2016 |
|
RU2642273C1 |
| KIT OF REAGENTS FOR GENETIC TYPING STRAINS OF ANTHRAX AGENT BY FRAGMENT ANALYSIS "OM-SIBERIAN PLAGUE-GENOTYPE" | 2019 |
|
RU2730868C1 |
| METHOD OF DIFFERENTIATING TYPICAL AND ATYPICAL STRAINS Yersinia pestis OF MEDIEVAL BIOVAR BY PCR METHOD WITH HYBRIDISATION-FLUORESCENT RECORDING RESULTS | 2014 |
|
RU2550257C2 |
| SET AND METHOD FOR ACCELERATED PLAGUE MICROBE IDENTIFICATION AND SIMULTANEOUS DIFFERENTIATION OF VIRULENT AND AVIRULENT STRAINS OF YPESTIS BY PLASMID PROFILING THEREOF | 2011 |
|
RU2473701C1 |
| METHOD FOR IDENTIFICATION OF YERSINIA PESTIS STRAINS OF A MEDIEVAL BIOVAR WITH SUBSEQUENT DIFFERENTIATION BY PHYLOGENETIC AFFILIATION BY POLYMERASE CHAIN REACTION WITH HYBRIDIZATION-FLUORESCENT ACCOUNT OF RESULTS | 2018 |
|
RU2705813C1 |
