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2 471 872

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IPC

C12Q1/68(2006-01-01)

C12N15/11(2006-01-01)

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Application

2011137371/10, 2011-09-08

(24)

Start date

2011-09-08

(22)

Actual filing date

2011-09-08

(45)

Published

2013-01-10

(72)

Inventor

Eroshenko Galina AleksandrovnaKukleva Ljubov' MikhajlovnaPavlova Alla IvanovnaOdinokov Georgij NikolaevichKrasnov Jaroslav MikhajlovichGuseva Natal'Ja PetrovnaKutyrev Vladimir Viktorovich

(73)

Holder

Rossijskaja Federatsija, Ot Imeni Kotoroj Vystupaet Federal'Naja Sluzhba Po Nadzoru V Sfere Zashchity Prav Potrebitelej I Blagopoluchija Cheloveka

METHOD FOR DIFFERENTIATING YERSINIA PESTIS STRAINS OF VARIOUS SUBSPECIES AND BIOVARS BY POLYMERASE CHAIN REACTION AND MULTILOCUS SEQUENCE TYPING Russian patent published in 2013 - IPC C12Q1/68 C12N15/11

Abstract RU 2471872 C1

FIELD: medicine.

SUBSTANCE: method provides: recovering chromosome DNA of the examined strain, conducting polymerase chain reaction with primers Nap469/Nap1187, rhaSc-s/rhaSc-a, glpD-Fl/glpD-Rl, aRaCF2/aRaCR2 with the primers of amplification genes of napA and rhaS gene fragments having the following nucleotide sequences: Nap469-TACGCGGCG TTGAAGTTG; Nap1187-TTGCCGGTT AACAGGTGC, rhaSc-s-GGTTTAGTCA TCACTGCTGC; rhaSc-a-GAAGTGCGGGAAAGAA, genetic typing by the presence of nucleotide substitutes in variable sites of the used genes, and sequence typing of the examined strain. The examined plague agent strains are differentiated by comparing their sequence types and the sequence types of the strains of primary and secondary subspecies and specific biovars of the plaque agent.

EFFECT: method enables effective and reliable intraspecific differentiation of the Yersinia pestis strains by subspecies and biovars.

1 dwg, 2 tbl

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RU 2 471 872 C1

Authors

Eroshenko Galina Aleksandrovna

Kukleva Ljubov' Mikhajlovna

Pavlova Alla Ivanovna

Odinokov Georgij Nikolaevich

Krasnov Jaroslav Mikhajlovich

Guseva Natal'Ja Petrovna

Kutyrev Vladimir Viktorovich

Dates

2013-01-10—Published

2011-09-08—Filed