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IPC

C12N1/00(2006-01-01)

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Application

2016130660, 2016-07-25

(24)

Start date

2016-07-25

(22)

Actual filing date

2016-07-25

(45)

Published

2018-01-24

(72)

Inventor

Trukhachev Aleksej LeonidovichSochivko Dmitrij GarrievichKonovalova Nina ValerevnaBlagodatskikh Konstantin AleksandrovichKuznetsova Darya Aleksandrovna

(73)

Holder

Federalnoe Kazennoe Uchrezhdenie Zdravookhraneniya Ordena Trudovogo Krasnogo Znameni Nauchno-Issledovatelskij Protivochumnyj Institut" Federalnoj Sluzhby Po Nadzoru V Sfere Zashchity Prav Potrebitelej I Blagopoluchiya Cheloveka

METHOD OF DIFFERENTIATING YERSINIA PESTIS STRAINS ON BASIC AND NONBASIC SUBTYPES BY PCR METHOD IN REAL TIME MODE Russian patent published in 2018 - IPC C12N1/00

Abstract RU 2642273 C1

FIELD: biotechnology.

SUBSTANCE: method of differentiating Yersinia pestis strains into basic and nonbasic subtypes by PCR method in the real-time mode is described. The method includes amplification of the tested DNA using engineered primers: ftKfor (5'-tcatcgttggtgtcagttgc-3'), ftsKrev (5'-tggcgatgatgacgttt-3') in the presence of allele-specific probes: ftsKProbaA (5'-Fam-aactggtacagaaAcaactgataca-RTQ1-3'), ftsKProbaG(5'-Hex-ggtacagaaGcaactgatacaaaa-BHQ2-3'), in the presence of fluorescent labels FAM and HEX and fluorescence quenchers RTQ1 and BHQ2.

EFFECT: arsenal of ways of differentiating strains of Yersinia pestis is expanded.

3 cl, 2 ex

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RU 2 642 273 C1

Authors

Trukhachev Aleksej Leonidovich

Sochivko Dmitrij Garrievich

Konovalova Nina Valerevna

Blagodatskikh Konstantin Aleksandrovich

Kuznetsova Darya Aleksandrovna

Dates

2018-01-24—Published

2016-07-25—Filed