FIELD: biotechnology.
SUBSTANCE: set of synthetic oligonucleotides is proposed for the detection of the Grapevine fleck virus (Grapevine fleck virus) using the polymerase chain reaction method. The proposed kit includes primers GFkV_F 5'-CCC-TCG-TGT-AAG-CAT-CCA-(C/T) CT-3' and GFkV_R 5'-AAG-ACG-GAG-AGG-AT(T/C)-TC(A/G)-GA-3' and allows to reliably detect the grape mottle virus in symptomatic and asymptomatic biological material of grapes.
EFFECT: kit can be used in molecular diagnostics of grape biomaterial for the presence of a grape mottle virus in laying new vineyards, during phytosanitary examinations and in laboratory diagnostics of samples.
1 dwg, 1 tbl, 1 ex
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