OLIGONUCLEOTIDE PRIMERS, DNA-PROBE AND METHOD FOR DETECTION OF VIRUS OF INFECTIOUS ANAEMIA IN HORSES BY METHOD OF PCR OR RT-PCR IN REAL TIME MODE Russian patent published in 2015 - IPC C12Q1/68 

FIELD: medicine.

SUBSTANCE: group of inventions relates to field of biotechnology. Method of detecting genome of virus of infectious anaemia in horses includes carrying out polymerase chain reaction, amplification of virus DNA, evaluation of reaction carrying out. If viral RNA is detected, reaction of denaturation and reverse transcription is preliminarily carried out, and detection is carried out by means of RT-PCR in real time in case of detection of INAN virus and PCR in real time in case of detection of pDAN of INAN virus. To realise the method specific primers and DNA-probe are applied. Temperature modes include the following stages: 5 min of preliminary denaturation at 94°C, 5 cycles of amplification without detection (94°C - 10 sec, 56°C - 15 sec, 72°C - 15 sec) and 35 cycles of amplification with detection (94°C - 10 sec, 56°C - 15 sec, 72°C - 15 sec). Interpretation of results is carried out on the basis of presence/absence of crossing of fluorescence curve with threshold line (Threshold).

EFFECT: application of inventions makes it possible to detect RNA and pDAN of INAN virus by means of RT-PCR in real time mode, as well as to increase method specificity and sensitivity.

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