FIELD: molecular biology.
SUBSTANCE: invention is a test system for detecting an apple stem pitting virus by real-time PCR. Test system includes: an amplification mixture consisting of a reaction buffer, deoxynucleotide triphosphates, two oligonucleotide primers specific to a gene fragment coding RNA-dependent RNA polymerase, one oligonucleotide probe labelled with a fluorescent FAM dye, positive control sample, which is a recombinant plasmid DNA containing the corresponding fragment of the virus genome.
EFFECT: development of a test system for highly specific identification of cDNA, with high sensitivity for detection of apple stem pitting virus ASPV by PCR method in real time.
1 cl, 4 dwg, 3 tbl
| Title | Year | Author | Number |
|---|---|---|---|
| TEST SYSTEM FOR DETECTION OF APPLE STEM GROOVING VIRUS BY REAL-TIME PCR METHOD | 2024 |
|
RU2835202C1 |
| TEST SYSTEM FOR DETECTION OF APPLE CHLOROTIC LEAFSPOT VIRUS BY REAL-TIME PCR METHOD | 2024 |
|
RU2835209C1 |
| TEST SYSTEM FOR DETECTING APPLE-TREE CANKER AGENT SPHAEROPSIS MALORUM BY REAL-TIME PCR | 2024 |
|
RU2840150C1 |
| TEST SYSTEM FOR REAL-TIME PCR DETECTION OF PHYTOPHTHORA CACTORUM ROOT COLLAR LATE BLIGHT AGENT | 2024 |
|
RU2838921C1 |
| METHOD OF EXTRACTION RNA OUT OF VEGETABLE SAMPLES | 2008 |
|
RU2389795C1 |
| METHOD FOR DIFFERENTIATION OF GENOME OF ARRIAH VACCINE STRAIN FROM FIELD ISOLATES OF RABIES VIRUS BY POLYMERASE CHAIN REACTION IN REAL TIME WITH ANALYSIS OF PEAKS OF MELTING TEMPERATURES OF AMPLICONS AND USE OF ASYMMETRIC DYE SYBR GREEN | 2023 |
|
RU2822037C1 |
| SET OF OLIGODEOXYRIBONUCLEOTIDE PRIMERS AND FLUORESCENT-LABELLED PROBE FOR JMTV RNA IDENTIFICATION BY PCR WITH HYBRIDISATION-FLUORESCENCE DETECTION | 2023 |
|
RU2818960C1 |
| SET OF OLIGONUCLEOTIDES AND METHOD FOR ENRICHMENT OF GENOMIC CDNA OF VIRUS OF MURINE CONGO HAEMORRHAGIC FEVER GENOTYPE EUROPA-1 (V) BY POLYMERASE CHAIN REACTION | 2023 |
|
RU2839313C1 |
| SET OF OLIGONUCLEOTIDE PRIMERS FOR DETECTING RNA OF YELLOW FEVER VIRUS BY ISOTHERMAL AMPLIFICATION IN REAL TIME | 2024 |
|
RU2824209C1 |
| SET OF OLIGONUCLEOTIDE PRIMERS AND FLUORESCENT-MARKED PROBERS FOR SUBCOMPOUND-SPECIFIC IDENTIFICATION OF RNA OF DENGUE VIRUS BASED ON MULTIPLEX PCR IN REAL TIME | 2012 |
|
RU2483115C1 |
