FIELD: biotechnology.
SUBSTANCE: method for obtaining of a polypeptide recombinantly produced in periplasm by prokaryotic cells is proposed. The method comprises prokaryotic cells incubation in a solution containing Tris at a concentration of 10 mM to 95 mM and EDTA at a concentration of 2 mM to 6 mM and EDTA, at a pH of 7 to 10 for 15 minutes to 6 hours at a temperature of 20°C to 33°C.
EFFECT: invention provides isolation of the recombinantly produced polypeptide in high yield.
6 cl, 2 dwg, 3 ex
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