FIELD: biotechnology.
SUBSTANCE: compositions and methods of displaying antibodies in the periplasmic space of yeast cells are described. Besides, the invention relates to high throughput screening of antibody libraries using periplasmic display in yeast cells. Antibodies are associated with a membrane-spanning transmembrane domain, a cell membrane-associated protein domain that resides on the outer surface of the yeast cell membrane, a protein that binds to the inner surface of the yeast cell wall, or a periplasmic protein to display antibodies in the yeast periplasmic space.
EFFECT: possibility to co-express a target protein of interest in yeast, so that it is localized on the plasma membrane or periplasmic space and is available for binding by exposed antibodies.
117 cl, 7 dwg, 2 tbl, 8 ex
| Title | Year | Author | Number |
|---|---|---|---|
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