FIELD: biotechnology.
SUBSTANCE: identification of citrate-assimilating enterobacteria isolated during microbiological diagnosis of dysbiotic conditions of the intestine (dysbacterioses) in children and adults is carried out. The method involves reseeding of the microorganisms colony grown on the Endo or Simmons medium, reseeding for primary and subsequent identification is performed using tests of indole formation, hydrogen sulfide production, mobility and sodium citrate utilisation. By the nature of the changes, the microorganisms studied are divided into six conditional groups at the beginning. In future, depending on belonging to a certain group, the main and, if necessary, additional test for each group is used, namely: lysine and, if necessary, urea for group I, including immobile bacteria that do not produce indole and hydrogen sulphide and assimilate sodium citrate when grown on the Simmons medium: Klebsiella pneumoniae with subspecies: ssp.pneumoniae, ssp.ozenae, ssp.rhinoscleromatis. Lysine and, if necessary, successively sorbitol, arginine and rhamnose - for group III, including mobile bacteria that do not produce indole and hydrogen sulphide and assimilate sodium citrate: Enterobacter cloacae ssp.cloacae, Enterobacter aerogenes, Serratia marcescens, Serratia plymuthica and Serratia rubidaea. Phenylalanine, and later sodium malonate - for group IV, including mobile strains producing indole and not producing hydrogen sulphide, as well as assimilating sodium citrate: Citrobacter amalonaticus, Citrobacter koseri, and bacteria of the Providencia genus. Phenylalanine and subsequently lactose - for group VI, including non-indole and hydrogen sulfide-producing bacteria, most of which assimilate sodium citrate: Proteus mirabilis, Citrobacter freundii, Citrobacter youngae. Group II and V bacteria are identified only by primary tests and include immobile indole-producing strains that do not produce hydrogen sulphide and assimilate sodium citrate: Klebsiella oxytoca (group II), as well as mobile strains producing indole and hydrogen sulphide and mostly assimilating sodium citrate: Proteus Vulgaris (group V).
EFFECT: invention allows to increase identification by determining the genus, species and subspecies of citrate-assimilating enterobacteria.
1 tbl
