METHOD OF THE VITAMIN K CONTENT DETERMINING IN PRODUCTS OF PLANT ORIGIN Russian patent published in 2018 - IPC G01N1/28 G01N30/02 

FIELD: measurement technology; biotechnology.

SUBSTANCE: invention relates to methods of the biologically active substances quantitative determination in plant raw material and food products derived therefrom, namely to a method of the vitamin K₁ content determining in the products of vegetable origin, and can be used in the chemical, pharmaceutical and food industries, medicine, including food hygiene. Method of the vitamin K₁ content determining in products of vegetable origin includes the vitamin K₁ (phylloquinone) quantitative extraction from the plant products with a propanol-2 and hexane mixture, separation of the obtained extract, elimination of the matrix components effect by filtering of the extract through a hydrophobic membrane, evaporation of the extract in a stream of nitrogen, the sample re-dissolution in the eluent and analysis by high-performance liquid chromatography with chromatography data mathematical processing, which allows to establish the absolute value of the phylloquinone concentration in 100 g of a plant origin product.

EFFECT: method allows to determine the vitamin K₁ concentration both without concentration process, and with 60-fold concentration with the use of a minimum amount of reagents and the minimum configuration of the analytical system, as well as in any of the plant origin products.

1 cl, 2 dwg, 8 tbl, 2 ex