FIELD: biotechnology.
SUBSTANCE: method includes: contacting of destroyed cells or mixtures of antigen-binding proteins with an affinity substrate with protein A, and elution of bispecific antigen-binding protein using a pH gradient in the presence of ionic modifier. Method includes application of an Ionic modifier, which is salt, selected from beryllium, lithium, sodium or potassium acetate; sodium or potassium bicarbonate; lithium, sodium, potassium or cesium carbonate; lithium, sodium, potassium, cesium or magnesium chloride; sodium or potassium fluoride; sodium, potassium or calcium nitrate; sodium or potassium phosphate; or calcium or magnesium sulfate.
EFFECT: invention expands the arsenal of means for antigen-binding bispecific proteins isolation.
25 cl, 11 dwg, 3 tbl, 12 ex
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