NON-HUMAN ANIMALS EXPRESSING PH-SENSITIVE IMMUNOGLOBULIN SEQUENCES Russian patent published in 2018 - IPC A01K67/27 C12N15/85

Abstract RU 2664473 C2

FIELD: biochemistry.

SUBSTANCE: invention relates to biochemistry, in particular to a genetically modified rodent for producing variable domains of immunoglobulin containing an amino acid histidine, encoded from its germline. Said rodent comprises in its germline an immunoglobulin locus that contains a non-arranged sequence of a variable immunoglobulin gene, comprising, in the sequence encoding the complementarity determining region 3 (CDR3), the replacement of at least one non-histidine codon with a histidine codon or insertion of at least one histidine codon. Also disclosed is a method for producing said rodent.

EFFECT: invention makes it possible to efficiently obtain domains of immunoglobulins that bind to antigens in a pH-sensitive manner.

47 cl, 29 dwg, 8 tbl, 9 ex

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RU 2 664 473 C2

Authors

Mcwhirter John

Macdonald Lynn

Martin Joel H.

Murphy Andrew J.

Dates

2018-08-17—Published

2013-03-15—Filed