ANTIBODIES WITH BUILT IN THE LIGHT CIRCUIT BY HYSTIDINE AND GENETICALLY MODIFIED EXCELLENT FROM HUMAN ANIMALS FOR THEIR OBTAINING Russian patent published in 2018 - IPC A01K67/27 

FIELD: biotechnology.

SUBSTANCE: rodents are designed to produce antibodies that contain a light chain of immunoglobulin with a human variable domain, in which the amino acid histidine is encoded by a substituted or inserted germline codon. To obtain such rodents, a modification of its embryonic genome is performed to remove or reduce the endogenous V and J segments of the light chain of immunoglobulin to a non-functional state. Thereafter, a single rearranged human immunoglobulin light chain variable region gene sequence is inserted into the genome of said rodent, comprising sequences of V_L and J_L human, and the gene sequence of the immunoglobulin light chain constant region operably linked to a single rearranged human immunoglobulin light chain variable region gene sequence. The immunoglobulin light chain is capable of forming a pair with a number of heavy chains of immunoglobulin selected from the rodent. Each of the plurality of heavy chains of immunoglobulin specifically binds to different epitopes.

EFFECT: invention makes it possible to obtain antibodies having more efficient recycling, to prevent antibody degradation by promoting the dissociation of antibody-antigen complexes in the endosomal compartment without reducing the specificity and affinity of the antibody to the antigen.

47 cl, 14 dwg, 6 tbl, 5 ex