FIELD: chemistry.
SUBSTANCE: invention relates to biochemistry and molecular biology, in particular to a set of synthetic oligonucleotides. Specified set is designed to determine the 1 intron sequence adjacent to the 2 exon of genes of the HLA I and II class system (HLA-A and HLA-DRB1) by the PCR method and consists of the following primers: dr1in6s 5’-GGATCCTCCTCCAGCTCCTG-3’, dr1in2a 5’-CCGCTCCGTCCCATTGAAGA-3’, A1in1s 5’-CTCCGAACCCTCCTCCTGCTA-3’, A1in2a 5’-GCTGTCGAACCGCACGGACTG-3’.
EFFECT: invention makes it possible to determine the sequences of the 1 intron adjacent to the 2 exon, the genes of the HLA system of classes I and II.
1 cl, 1 dwg
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