METHOD FOR SEQUENCING EXONS OF HLA-DQA1 GENE AND SET OF SYNTHETIC OLIGONUCLEOTIDES FOR ITS IMPLEMENTATION Russian patent published in 2025 - IPC C12Q1/00 

FIELD: biochemistry; molecular biology.

SUBSTANCE: disclosed is a method for sequencing exons of the HLA-DQA1 gene by fragmentary NGS sequencing, involving the following steps: (a) extraction of DNA from biological material; (b) performing PCR using oligonucleotide primers having the structure SEQ ID NO: 1-8, wherein the reaction mixture for amplification contains oligonucleotide primers in the following concentration: SEQ ID NO: 1, 2, 3, 4, 7, 8 – 0.1 pmol/mcl each; SEQ ID NO: 5, 6 – 0.2 pmol/mcl each; (c) purifying PCR products using magnetic particles; (d) indexing the obtained amplicons; (e) repeated purification of PCR products using magnetic particles. Disclosed is a set of oligonucleotide primers for implementing the method according to cl. 1, having the structure SEQ ID NO: 1-8, to perform the function of primers complementary to HLA-DQA1 gene regions.

EFFECT: invention enables sequencing exons of the HLA-DQA1 gene and determining the alleles of the given gene contained in the initial sample based on the sequencing results, as well as eliminating the amplification of non-target regions of human DNA.

4 cl, 1 tbl, 4 ex