NOVEL METHOD FOR PCR-SEQUENCING AND USE THEREOF FOR HLA GENOTYPING Russian patent published in 2016 - IPC C12Q1/68 C12N15/11

Abstract RU 2587606 C2

FIELD: biochemistry.

SUBSTANCE: invention relates to biochemistry. Described is method of polymerase chain reaction sequencing, involving following steps: obtaining sample; amplification; mixing; fragmentation; sequencing and splicing. Present invention also relates to primer marks used in above method, as well as to method of genotyping, in particular, in analysis of HLA. Present invention also relates to PCR primers for HLA-A/B, HLA-C and HLA-DQB1.

EFFECT: invention allows to increase length of the PCR product, which can be sequenced by sequencer, without increasing price and/or reduction.

19 cl, 18 dwg, 13 tbl, 12 ex

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RU 2 587 606 C2

Authors

Li Tszyan

Chen Shipin

Chzhan Syandun

Lyu In

Chzhan Tsajfen

Lyu Tao

Chzhao Mejzhu

Dates

2016-06-20—Published

2011-06-30—Filed